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Thread: Fish Experiment

  1. #1 Fish Experiment 
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    May 2013
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    1) Which DNA should I use to determine hybridization? I was thinking to isolate mtDNA and run that, then isolate nuclear DNA and run that as well. I read that mtDNA is not a sufficient determinance for hybridization, that's why I will also be using nuDNA. 2) What procedures whould I use to isolate nuDNA from mtDNA? I was told to use CHELEX to isolate the nulcear DNA, but CHELEX appears to not differentiate between the nucleic acids.


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