So, I have a medical theory that I am wanting to test, particularly with the use of bacterial and human cells. Problem is, aside from a microscope, I know not what materials I need to acquire for my experiment. I conjecture that to receive proper instruction on such, I will need to speak of my intentions for the experiment. So here goes: I plan on damaging the genetic material of bacterial cells (likely E. Coli) using ultraviolet radiation. Then, the bacterial cells with damaged nucleic acids will be exposed to the human (animal) cells. My questions concerning the experiment are as follows:
- Will ultraviolet radiation be enough to damage the bacteria's genetic material after an exposure time of six hours?
- What would be the optimal way to expose the bacteria to the animal cells so that I could view any change in the animal cells?
- Should I acquire an incubator for this experiment to optimize reproduction of the bacterial cells?
Thanks!