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View Poll Results: My situation is:

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  • Dangerous

    0 0%
  • Normal coz you washed them

    1 100.00%
  • Don't know, not sure about that

    0 0%
Results 1 to 5 of 5

Thread: Safety: Taq Polymerase contamination!!

  1. #1 Safety: Taq Polymerase contamination!! 
    New Member
    Join Date
    May 2011
    Posts
    1
    Hi everyone,

    I was contaminated by the master mix of PCR reaction: Taq polymerase, primers, dNTPs, ... I washed them immediately by alcohon 70%. Just a very small amount of them, but it makes me so worried. Is there any influence? How do we deal with that?

    Thanks so much


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  3. #2  
    Forum Professor
    Join Date
    Sep 2007
    Posts
    1,079
    There may be exponentially more of you within 30 cycles.


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  4. #3  
    Moderator Moderator TheBiologista's Avatar
    Join Date
    Aug 2008
    Posts
    2,564
    It wouldn't have done anything to you even if you hadn't washed the mixture off. It's harmless.
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  5. #4  
    New Member
    Join Date
    Nov 2012
    Posts
    2
    Hi
    Can you tell me if the PCR reaction mixture containing the DMSO,Enhancer are harmful on skin contact in minute amount.I washed within 20 mins of skin contact.Is there anything to worry about
    I appreciate Your valuable comments
    Thanks
    Karmel
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  6. #5  
    Forum Freshman
    Join Date
    May 2009
    Posts
    5
    You may want to keep monitoring any problems that may occur and report to your Dr right away.
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