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Thread: Endothelial dysfunction

  1. #1 Endothelial dysfunction 
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    I am doing hypertension research, and mostly test the function of aortic ring. I am facing some problem when i run the experiments. Sometimes, the aortic ring not being contracted by phenylephrine(PE, 10-7M; g tension=0.3-0.45g) but it's contracted by high K(KCl,80mM;>0.55g). Why? Please Help. Thanks!


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  3. #2  
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    Why are you even using phenylephrine to induce hypertension? Why not just find a native population?

    And why ask this question on a science forum, surely your peers would be better placed to answer?

    Don't know enough about the cardiovascular system to give a full answer, but i'd hazard a guess at either the aorta not being particularly rich in alpha-1 receptors or genetic mutations in some individuals along the phenylephrine pathway mean some won't respond the same as others.

    Also, that's a massive dose of potassium, how are you giving it without endangering people? Are these animal experiments?


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  4. #3  
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    Thanks for your reply.

    I am not using PE or KCl to induced hypertension to rats. All my experiment is in vitro....i abstract out the rats' aorta, then i test on it with the drugs. Anyway, i think i should consider your answer about the density of alpha-receptor in the aortic....it should be one of the problems too....

    Thanks!
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