Notices
Results 1 to 6 of 6

Thread: A tricky cell structure from friut fly brain section

  1. #1 A tricky cell structure from friut fly brain section 
    New Member
    Join Date
    Nov 2008
    Location
    Beijing, China
    Posts
    3
    We sectioned a type of transgene fruit fly which is contaning the human a beta 42 gene on the 36days after ecolsion. And the H/E stainning was operated for observing the vacuoles. I noticed there was a kind of cell looked quiet different with other type cells. the bound of the nucleoclu was expanded, looking as a eye. I donot know what causes this structure, is it a processing apoptosis cell? or caused by a beta 42?

    Anyone interested in this, i can send the pictures to you and i wish there will be more further discussion with you.


    Say that is the way to go.
    Reply With Quote  
     

  2.  
     

  3. #2  
    Forum Ph.D.
    Join Date
    Aug 2006
    Posts
    792
    Could you tell me more about what exactly you're exploring with beta 42 and what this experiment was?
    Assuming this was in a neurodegenerative/Alzheimer's type of area.


    Reply With Quote  
     

  4. #3  
    Forum Cosmic Wizard i_feel_tiredsleepy's Avatar
    Join Date
    Mar 2008
    Location
    Montreal
    Posts
    2,256
    Is the histology of fruit fly brains similar to that of mammalian brains? Otherwise I'd have no idea what I was looking at lol.
    Reply With Quote  
     

  5. #4  
    New Member
    Join Date
    Nov 2008
    Location
    Beijing, China
    Posts
    3
    Yes, the flies expressed human wide type a beta 42 in brain, especially in the cell body of mushroom body, where is named as Kenyon cell layer. the vacuole is the result of neurodegeneration. And the area of vacuoles normally increase day by day after the eclosion day. we feed those flies with hundurd of potential compounds to asset the function of these compounds have curing effects or not.

    And H/E is a kind of stainning method.
    Say that is the way to go.
    Reply With Quote  
     

  6. #5  
    Forum Cosmic Wizard i_feel_tiredsleepy's Avatar
    Join Date
    Mar 2008
    Location
    Montreal
    Posts
    2,256
    Ya I'm pretty sure Robbie knows what an H/E stain is.

    Can we see a picture of the cell.

    I'm not overly familiar with fruit fly histology though.
    Reply With Quote  
     

  7. #6  
    New Member
    Join Date
    Nov 2008
    Location
    Beijing, China
    Posts
    3
    Colleagues, i found one paper including the same pic of what i found. they said this kind of stomata is the early stage of cell lysis.



    Katia Carmine-Simmen a, Thomas Proctor etc. (2008). Neurotoxic effects induced by the Drosophila amyloid-β peptide suggest a conserved toxic function. Neurobiology of Disease. YNBDI-01771; no of page: 8; 4C:3

    In Fig.4. F
    Say that is the way to go.
    Reply With Quote  
     

Bookmarks
Bookmarks
Posting Permissions
  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •