Thread: A tricky cell structure from friut fly brain section

We sectioned a type of transgene fruit fly which is contaning the human a beta 42 gene on the 36days after ecolsion. And the H/E stainning was operated for observing the vacuoles. I noticed there was a kind of cell looked quiet different with other type cells. the bound of the nucleoclu was expanded, looking as a eye. I donot know what causes this structure, is it a processing apoptosis cell? or caused by a beta 42?

Anyone interested in this, i can send the pictures to you and i wish there will be more further discussion with you.

Could you tell me more about what exactly you're exploring with beta 42 and what this experiment was?
Assuming this was in a neurodegenerative/Alzheimer's type of area.

Is the histology of fruit fly brains similar to that of mammalian brains? Otherwise I'd have no idea what I was looking at lol.

Yes, the flies expressed human wide type a beta 42 in brain, especially in the cell body of mushroom body, where is named as Kenyon cell layer. the vacuole is the result of neurodegeneration. And the area of vacuoles normally increase day by day after the eclosion day. we feed those flies with hundurd of potential compounds to asset the function of these compounds have curing effects or not.

And H/E is a kind of stainning method.

Ya I'm pretty sure Robbie knows what an H/E stain is.

Can we see a picture of the cell.

I'm not overly familiar with fruit fly histology though.

Colleagues, i found one paper including the same pic of what i found. they said this kind of stomata is the early stage of cell lysis.



Katia Carmine-Simmen a, Thomas Proctor etc. (2008). Neurotoxic effects induced by the Drosophila amyloid-β peptide suggest a conserved toxic function. Neurobiology of Disease. YNBDI-01771; no of page: 8; 4C:3

In Fig.4. F