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Thread: restriition mapping

  1. #1 restriition mapping 
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    Aug 2014

    Im trying to clone a fragment digested with EcoRI and SalI to pBluesript. Then sublone another fragment digested with SamI and EcoRI to the previous construct. To subcloned this latest fragment I have to digest the inicial fragment+vector with EcoRI and SamI.

    Im trying to make a direccional cloning and I have doubts in my restrition map...and if my strategy of cloning is correct.


    Andreia Silva (master degree student at Lisbonīs University).

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