Thread: restriition mapping

Hi,

Im trying to clone a fragment digested with EcoRI and SalI to pBluesript. Then sublone another fragment digested with SamI and EcoRI to the previous construct. To subcloned this latest fragment I have to digest the inicial fragment+vector with EcoRI and SamI.

Im trying to make a direccional cloning and I have doubts in my restrition map...and if my strategy of cloning is correct.

Thanks,

Andreia Silva (master degree student at Lisbon´s University).