Notices
Results 1 to 1 of 1

Thread: restriition mapping

  1. #1 restriition mapping 
    New Member
    Join Date
    Aug 2014
    Posts
    2
    Hi,

    Im trying to clone a fragment digested with EcoRI and SalI to pBluesript. Then sublone another fragment digested with SamI and EcoRI to the previous construct. To subcloned this latest fragment I have to digest the inicial fragment+vector with EcoRI and SamI.

    Im trying to make a direccional cloning and I have doubts in my restrition map...and if my strategy of cloning is correct.

    Thanks,

    Andreia Silva (master degree student at Lisbonīs University).


    Reply With Quote  
     

  2.  
     

Similar Threads

  1. Mapping
    By rock in forum General Discussion
    Replies: 1
    Last Post: October 14th, 2011, 09:01 AM
  2. Memory Mapping
    By Bad_Azz_Dude in forum Computer Science
    Replies: 0
    Last Post: December 6th, 2009, 01:03 AM
  3. Mind mapping
    By newnothing in forum General Discussion
    Replies: 0
    Last Post: July 28th, 2009, 10:55 AM
  4. Genome Mapping
    By gottspieler in forum Biology
    Replies: 6
    Last Post: December 31st, 2008, 01:04 PM
  5. Closed Mapping - Non-Linear Programming
    By holysword in forum Mathematics
    Replies: 8
    Last Post: April 30th, 2008, 12:18 PM
Bookmarks
Bookmarks
Posting Permissions
  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •