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Thread: Molecular biology and genetics (first year) homework problem: converting between #moles and concentration

  1. #1 Molecular biology and genetics (first year) homework problem: converting between #moles and concentration 
    New Member
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    Aug 2014
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    Hello, i am stuck on a problem for my molecular biology and genetics lab. if someone could help out by suggesting an approach method it would help a lot.

    Question: A reaction has been designed to be carried out in a 5ml total volume. it requires a final ATP (mol. wt.=350) concentration of 5mM. if the protocol allows 100 micro litres for the ATP addition, what concentration would you make the stock solution.

    even if you can't do it, i would like to know how you would attempt to approach it. that may help trigger a thought and send me on the right track.

    Thanks in advance.


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  3. #2  
    Forum Sophomore
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    Feb 2014
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    It doesn't seam very hard. But i really don't remember the moll thing how is done.... A long time ago

    If i understood corectly

    basically, you need a concentration of 5mM ATP in the total volume of 5ml
    From there you deduce what is the real quantity of ATP. You need to do correctly the unit conversions.

    That quantity must be present in just 100 micro litres of the solution you'll add
    you deduce the concentration.

    The real trouble is the conversions between units.
    I did chemistry a long time ago by the way....


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