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Thread: Improving the sharpness of protein bands

  1. #1 Improving the sharpness of protein bands 
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    I have been running SDS PAGE for protein sample, but I m not getting the sharp bands, is there is way how to improve the sharpness of the bands?


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  3. #2  
    Moderator Moderator Cogito Ergo Sum's Avatar
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    Have you consulted this page?
    You might find the answer there: Western blot troubleshooting tips


    "The only safe rule is to dispute only with those of your acquaintance of whom you know that they possess sufficient intelligence and self-respect not to advance absurdities; to appeal to reason and not to authority, and to listen to reason and yield to it; and, finally, to be willing to accept reason even from an opponent, and to be just enough to bear being proved to be in the wrong."

    ~ Arthur Schopenhauer, The Art of Being Right: 38 Ways to Win an Argument (1831), Stratagem XXXVIII.
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