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Thread: HELP!! Resuspended Anti-GFP Antibody in SDS!!!

  1. #1 HELP!! Resuspended Anti-GFP Antibody in SDS!!! 
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    Oct 2012
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    HELP!!

    One of the students in our lab accidentally resuspended our 200ug Anti-GFP antibody in 500uL of 20% SDS solution instead of sterile water!! (The bottles were right next to each other)

    What should I do from here??
    As the lab manager, I am responsible for all of the reagents, and antibodies are way too expensive to just order more!

    Some possibilites:
    1. Use them as is (will the antibodies even work now?)
    2. Use lyophilization to dry out the samples in our vacuum/centrifuge. However, I'm afraid the SDS will precipitate with the antibody.

    Any other ideas??


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