Results 1 to 1 of 1

Thread: freeze clamp as camera?

  1. #1 freeze clamp as camera? 
    New Member
    Join Date
    May 2012

    I'd like to discuss this idea (on youtube on the link below) to record which molecule(s) is(are) interacting with which ones when two kind of cells met. So far, in any case, at least one of the partner has to be know. The game then consist to try to identify who is binding to it (blotting, screening, labeling). Most of the time, it required firstly purification of known proteins. Here the idea is toreverse the mapping of cell-cell, cell-protein and protein-protein interaction by freezing, linking them with UV and then sequence the attached parts after isolation to identify the interacting partners. I use freeze clamp to freeze ionic channel so, they can be labeled with photoactive probes.
    My hypothesis is to use photoactivatable probes in the media where are the cells. When they become spatially very close to each other (example during an invasion), at their interfaces, some probes will be trapped. If the surfaces are complementary geometrically, the bond will be stronger (?). When the media is instant frozen, each partner will stay in its exact position at a precise moment of the molecular events happening. Nitrogen freezing enables the fast freezing. So, if the arms of the probe is close enough of each surface molecules, the UV irradiation will bind them together covalently. If the trio (part of cell A, part of cell B and the photoactive linker) are labeled with specific "dyes" or labels (radio-elements?), the all process will be to track any band on gel having the three signals. These trio bands having the label of the three markers will be purify and sequenced (liquid or Mass spec). The obtained sequence enables the identification of the partner in data base. Therefore, no need to know the interacting partners before sequencing them...
    Thank you for your enlightenment


    link youtube:

    Reply With Quote  


Similar Threads

  1. Replies: 5
    Last Post: October 23rd, 2011, 01:20 PM
  2. Why spraying H2O on plants is crucial during a freeze
    By NADH dehydrogenase in forum Biology
    Replies: 12
    Last Post: January 16th, 2010, 10:17 AM
  3. freeze drying
    By luci in forum Physics
    Replies: 8
    Last Post: May 16th, 2008, 12:00 PM
  4. racing games freeze with a loud beep with 2 video cards
    By Bloodling in forum Computer Science
    Replies: 1
    Last Post: July 4th, 2007, 11:56 PM
  5. camera replacing eye
    By anand_kapadia in forum Biology
    Replies: 6
    Last Post: June 11th, 2006, 07:47 PM
Posting Permissions
  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts