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Thread: PCR product size of CYP2A6

  1. #1 PCR product size of CYP2A6 
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    Mar 2012
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    I am working on the genotype of CYP2A6*1A, *1B and *4. I used the primers:

    FP: 5-CACCGAAGTGTACCCTATGCTG-3 and.

    RP: 5-AAAATGGGCATGAACGCCC-3.

    From ensemble, I have got the theoretical PCR product size 1320 bp. But from previous published papers I found that PCR product size is 1338 bp. How can I solve the problem without direct sequencing? Please help me for this problem.


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