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Thread: calculation help

  1. #1 calculation help 
    Forum Freshman
    Join Date
    Jul 2008
    I am staining some cells with antibodies for FACS analysis.
    my primary ab conc is 0.2ug/ul.

    In one tube i have 1,000,000 cells (the approx volume of these cells is 40ul).i add 1ul of my primary ab to these cells and run my facs
    If i have 10,000,000 cells (which is say 100ul of cells). How much of my antibody (ul) should i add to my 10,000,000 cells.

    Is it 10ul that i add. Do i need to take into account the volume of my cells?

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