Hey all
Currently I am doing a prac writeup on UV spectroscopy, and have a few questions that I have to answer:
Why would you use standard curves in assays as opposed to the Extinction ccoefficient? (assume you have access to it)?
I'm thinking that the Extinction coefficient is probably very dependant on experimental conditions etc.
Why might the slope of the standard curve disagree with the extinction coefficient?
Any help would be greatly appreciated.
Thanks