Thread: A Stupid Question

Will do an experiment tomorrow to know how much vitamin C is in a bottle of soft drink.

Will use the internal standard method of chromatography.

It states in the instructions that I have to make a solution which has vitamin C and isonicotinic acid in. Both of them have to have concentration of 10 ppm. This solution is my internal standaard.

So, if I understand well, I have a solution of 10 ppm vitamin C and a solution of 10 ppm isonicotinic acid in a flask. How the heck can I calculate the concentration of the solution?

perhaps if you titrate it against a standard solution you will find its concentration.
probably something like 0.1M NaOH. find the endpoint and work backwards to find the concentration

Use this method for preparing any standard solution.

Start with a solution of known concentration. Perhaps you prepared it yourself from the chemicals on the shelf. Then use successive dilutions to achieve the concentration you want.

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