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Thread: A Stupid Question

  1. #1 A Stupid Question 
    Forum Sophomore
    Join Date
    Jan 2008
    Le Ch√Ęteau de Moulinsart
    Will do an experiment tomorrow to know how much vitamin C is in a bottle of soft drink.

    Will use the internal standard method of chromatography.

    It states in the instructions that I have to make a solution which has vitamin C and isonicotinic acid in. Both of them have to have concentration of 10 ppm. This solution is my internal standaard.

    So, if I understand well, I have a solution of 10 ppm vitamin C and a solution of 10 ppm isonicotinic acid in a flask. How the heck can I calculate the concentration of the solution?

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  3. #2  
    Forum Masters Degree organic god's Avatar
    Join Date
    Feb 2008
    perhaps if you titrate it against a standard solution you will find its concentration.
    probably something like 0.1M NaOH. find the endpoint and work backwards to find the concentration

    everything is mathematical.
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  4. #3  
    Forum Bachelors Degree
    Join Date
    May 2007
    NC USA
    Use this method for preparing any standard solution.

    Start with a solution of known concentration. Perhaps you prepared it yourself from the chemicals on the shelf. Then use successive dilutions to achieve the concentration you want.

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