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Thread: enzyme purification. can anyone help me with this question?

  1. #1 enzyme purification. can anyone help me with this question? 
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    Join Date
    Sep 2007
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    An enzyme sample contains 24 mg protein/mL. Of this sample, 20 microliters in a standard incubation volume of 0.1 mL catalyzed the incorporation of glucose into glcogen at a rate of 1.6 nmol/min. Of this sample, 50 mL were fractionated by ammonium sulfate precipitation. The fraction precipitating between 30 and 50% saturation was redissolved in a total volume of 10 mL and dialyzed. The solution after dialysis had 12 mL and contained 30 mg protein/mL. Of the purified fraction, 20 microliters catalyzed the reaction rate of 5.9 nmol/min under the standard assay conditions. calculate the following:

    A. The recovery of enzyme after the ammonium sulfate step.
    B. The fold purification after the ammonium sulfate step.


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