I'm a new student of the course biochemistry. We had this topic about buffers a while ago. But I didn't understand it clearly. Can you guys help me answer this question:
A catalogue in the laboratory has a procedure to prepare 1L of a TRIS (N-tris [hydroxymethyl] aminomethane) buffer at 0.0500 M with a pH of 8. Dissolve 2.02 g of TRIS (free base, Molar Mass=121.1 g/mole) and 5.25 g of TRIS hydrochloride (acidic form, Molar Mass=157.6 g/mole) in a total volume of 1L. Verify if this procedure is correct.
That seems confusing to me, big time. Hehe. I'd appreciate it if you could help me. Thanks in advance. :-D