1. Hi im sorry i dont know if this is the correct forum to ask this question in but here it is:
I need to calculate the riboflavin content for a solution. It was made from 1 vitamin tablet dissolved in 20ml of water. Then the solution was made up to 100ml with neutral buffer. Then a 1:50 dilution was made with the solution. The concentration of riboflavin in the 1:50 solution was 86 μg/L. I need to know the riboflavin content in the tablet, in mg. Could anyone be kind enough to help me with this please. Thank you.

2.

3. Before the 50X dilution the concentration would be

50 x 86 = 4300 ug/L

As the sample was made up of 100 ml pre dilution this would contain 430ug. So your tablet contains 430ug of riboflavin.

4. so from what i understand the concentration of riboflavin would be 430 ug in 100ml of solution? which is basically 4300ug/L ?

5. Yes, you dissolved the tablet in 20 ml and then made it up to 100 ml. So the concentration is 430 / (100/1000) = 4300 ug/L. You then diluted this by a factor of 50 to give a final concentration of 4300/50 = 86 ug/L.

6. thank you very much for your help

7. No problem.

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