I am working on a microbiology lab write up and can't seem to figure out how KCl would effect a certain trial for a Gram positive bacterium cell when lysozymes were added. I understand that the lysozymes cut the peptidoglycan of the Gram positive cell. If the certain cell has an osmolarity of around 800 mOsm and the KCl makes the solution approximately 450 mOsm would the water rush in, out, or stay the same (hypotonic, hypertonic, isotonic)? My cells did not burst but I assumed they should have?
Thanks for your help