Thread: Biuret test

Is the Biuret test the chem test for protein? What is it and how do you know if there is protein or not?

Also, anyone have any good sites on tests for glucose, starch, fats, etc??????

I got this from wikipedia:

Biuret test is used for determining the amount of protein in a food sample. The Biuret test relies on the reaction between copper ions and peptide bonds in an alkaline solution. A violet color indicates the presence of proteins. Proteins give a strong biuret reaction because they contain a large number of peptide bonds. It is possible to use the biuret reaction to determine the concentration of proteins because (for most proteins) peptide bonds occur with approximately the same frequency per gram of material.

hehe did you write wikipedia

yes ... we ALL did