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Thread: Methyl group on DNA

  1. #1 Methyl group on DNA 
    Forum Sophomore Dkav's Avatar
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    There is a picture in my biochem book with a methyl group hanging off the thymine. What methylated the thymine? and is it only thymine that gets the CH3? Thanks...


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  3. #2  
    Forum Cosmic Wizard i_feel_tiredsleepy's Avatar
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    Usually it is cytosine that gets methylated, I'm not familiar with methylated thymine. Although, perhaps you confused the structures of uracil and thymine, since methylated uracil is thymine.


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  4. #3  
    Forum Sophomore Dkav's Avatar
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    Quote Originally Posted by i_feel_tiredsleepy
    Usually it is cytosine that gets methylated, I'm not familiar with methylated thymine. Although, perhaps you confused the structures of uracil and thymine, since methylated uracil is thymine.
    In Lehninger "Bible" of Biochemistry a.k.a. life, CH3 is on the thymine, figure 8-11. Also check out this model of thymine.
    http://en.wikipedia.org/wiki/File:Thymine-3D-balls.png
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    Forum Sophomore Dkav's Avatar
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    So im just wondering what its function is on the thymine?
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  6. #5  
    Forum Sophomore Dkav's Avatar
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    found this...looks like it has to do with structure http://pubs.acs.org/doi/abs/10.1021/jp911055x
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  7. #6  
    Forum Sophomore Dkav's Avatar
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    Ok, I answered my own question, here is another study http://www.jbc.org/content/277/32/28491.long
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  8. #7  
    Forum Cosmic Wizard i_feel_tiredsleepy's Avatar
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    The C5 methyl group is part of the structure of thymine, it's not added to thymine. If you remove that methyl group you get uracil.

    I thought you meant some additional methylation, like what occurs with cytosine during DNA methylation, which is a major component of DNA regulation.
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  9. #8  
    Forum Sophomore Dkav's Avatar
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    Quote Originally Posted by i_feel_tiredsleepy
    The C5 methyl group is part of the structure of thymine, it's not added to thymine. If you remove that methyl group you get uracil.

    I thought you meant some additional methylation, like what occurs with cytosine during DNA methylation, which is a major component of DNA regulation.
    Wow ok I see now. Thank you for the clarification.
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  10. #9  
    Forum Professor Zwirko's Avatar
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    For the record, the methyl group got there by the action of the thymidylate synthase enzyme; the methyl group itself is donated by 5,10-methylenetetrahydrofolate.
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