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Thread: Basic Chemistry Ambiguity

  1. #1 Basic Chemistry Ambiguity 
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    Jun 2010
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    Ok, I started a new internship in a lab for the first time and havent taken chemistry in years, so needless to say im having a stupid moment. I was told to make a 300mL preparation of LB medium from a recipe that says 10g tryptone, 5g yeast extract, and 5 g NaCl per liter. I multiplied all of the values by .3 to get 3 g tryptone, 1.5g yeast extract, and 1.5g NaCl. Did I do this right? O.o


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  3. #2  
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    That seems right, there's 1000 ml per liter and the recipe is by liter. You want .3 liters so .3 of everything in your recipe seems right. But honestly, I'd ask someone to make sure.


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  4. #3  
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    remember that in chem, its always proportional.

    so yes, your right
    I'm a refugee from /sci/
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  5. #4  
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    ^^ Yea, I was just paranoid cause I havent covered this material in a long time and remember some of the headaches in chem involving molarity calculations and the like. I had to do a molar calculation later in the day though

    Thanks for the feedback!
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