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Thread: TLC(Thin Layer Chromatography

  1. #1 TLC(Thin Layer Chromatography 
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    What will happen if there is an overrun of solvent in TLC where the solvent front reaches the top to the TLC plate?
    Can you still obtain an accurate Rf value?


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  3. #2  
    Forum Ph.D.
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    I think that if it was taken out of the solvent tank at the exact moment as it reached the top of the plate, then it should be grand.

    If it was left longer as you said it was, I donít think it would be accurate and you'd be better running a second. If itís a real experiment, you definitely should run a second if you can.

    (Forgive me for saying this, but if itís a homework question, I donít think I answered it for you, best to consider why you stop it at a certain point and what effect it has and hence what the Rf ratio actually means.)


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  4. #3  
    Forum Masters Degree samcdkey's Avatar
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    Since the Retention of your component is calculated relative to the distance the solvent front has travelled, I would suggest an overrun would overestimate the Rf.
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  5. #4  
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    .......although if it's just a routine test to see if your material is there and it's been used to gauge a known spot from previous test .....it doesn't matter if the solvent over runs.... it only matters if you're seeking and want to read the rf of your material....solo
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