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Thread: Phototoxtest with 3T3

  1. #1 Phototoxtest with 3T3 
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    Join Date
    Jan 2009
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    1
    I have a problem with the phototox test on the 3. day with the NR-colouration.
    In the OECD guideline 432 the NR-colouration only allows a NR-colouration with DMEM-medium without NCS-serum.
    I have the problem, that the cells then don`t take up enough NR (low extinction). Many of the cells die also. When I incubate with NR-medium with NCS I have a high extinction and all cells are alive, but this isn`t conform with the OECD guideline.
    Has anybody a solution of this problem?
    Thank you
    Sabine


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