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Thread: A Couple of Questions: Isoelectric Point and Extraction

  1. #1 A Couple of Questions: Isoelectric Point and Extraction 
    Forum Sophomore
    Join Date
    Jan 2008
    Le Ch√Ęteau de Moulinsart
    I currently have a project. It's a combination among some fields. Because I don't know much about Chemistry and know only something about Biochemistry, I think I ask these here.

    1. Take alanine for example. It has an isoelectric point of 6. Let's do in in a solution which has a pH of 10 and let's add concentrated sulfuric acid into the solution. Would I get my alanine back when the pH reaches 6 and could I separate it by evaporating the liquid?
    2. I have a solution of phenylalanine and phenylalanine amide in water. If I separate them through extraction, would amide still be in water which results from the extraction?

    Thanks much. [/list]

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