Extraction of Catalase

**darmic** · March 25th, 2008, 04:44 AM

Hi,

Can anyone please tell me how I can extract catalase from a rabbit's liver if it is a possible process?

thanks

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**i_feel_tiredsleepy** · March 25th, 2008, 09:07 AM

I don't know about isolating from animal tissue, there are plenty of procedures for isolating from plants available online. You can buy kits to assay catalase activity though.

Edit: In microbiology labs we just drop peroxide on the bacteria to see if they have catalase or not lol.

**CShark** · May 9th, 2008, 01:33 PM

You must be a med tech, correct ? Where did you go to school ?

Got my R.T from Algonquin

**free radical** · May 9th, 2008, 02:09 PM

Originally Posted by darmic

Hi,
Can anyone please tell me how I can extract catalase from a rabbit's liver if it is a possible process?

it should be possible. Ex from pigs:

http://www.jstor.org/pss/3627031

The procedure should be similar. Did you try Google.

**i_feel_tiredsleepy** · May 9th, 2008, 02:14 PM

Originally Posted by CShark

You must be a med tech, correct ? Where did you go to school ?

Got my R.T from Algonquin

No I'm working on my bachelor's degree in Microbiology and Immunology at McGill University, but I've taken the laboratory course in medical microbiology so I'm familiar with the tests for diagnosis and identification of pathogens.

**CShark** · May 9th, 2008, 02:43 PM

I see. The med lab micro course is three years, two theory and labs, and one clinical.

**i_feel_tiredsleepy** · May 9th, 2008, 02:48 PM

Originally Posted by CShark

I see. The med lab micro course is three years, two theory and labs, and one clinical.

Ya I've taken two year long lab courses and and a ton of theory, I won't do clinical obviously though. The lab course for me was more designed to become familiar with the techniques for studying viruses, bacteria, and human immune cells. The diagnosis section I think they put in just to fill space lol. I have a parasitology lab next year too.