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Thread: Shell-less CAM assay

  1. #1 Shell-less CAM assay 
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    Hello,

    I am currently trying to get a CAM assay up and running in our lab. I am planning to compare the traditional "in-shell" assay to the newer "shell-less" assay in 100mm dishes. Yesterday was day 3 so I tried to open 12 eggs into the dishes with an approximately 50% success rate. My best method was to use a scalpel over the air sac, cut a hole, flake away enough shell for the contents to be able to pass, and then to gently pour the contents into the dish. Sometimes this still resulted in "leaking" of the yolk from it's membrane.

    I will be making my "windows" for the traditional method on Monday.

    If anyone has a protocol or even tips to increase my success rate, that would be greatly appreciated. Literature reviews have not been helpful, they just mention that the assay was carried out in this method, not how they got to that point.

    Thanks everyone!


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  3. #2  
    Forum Ph.D. Wolf's Avatar
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    You might get an answer here, but to be honest yer best bet is probably here:

    Tech Forums

    The folks there should be able to help...but...uh...mind you don't step in any of their bullhocky while yer there. :-D


    Wolf
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    "Be fair with others, but then keep after them until they're fair with you." Alan Alda
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  4. #3  
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    Thanks! It appears that the forum you linked is a computer forum. Are there people there that are knowledgeable of angiogenic assays?
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  5. #4  
    Forum Ph.D. Wolf's Avatar
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    Somewhere in there. I haven't been on that forum in a long time, but there's all walks of folks there, from mom-n-pop mac users, to network gurus.

    Anyway, yer chances are better over there than here, I'm guessing.

    Edit: On second thought (and another cup of coffee) you and I are on two completely different pages. Excuse me while I turn beat red and stop posting...
    Wolf
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    "Be fair with others, but then keep after them until they're fair with you." Alan Alda
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