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Thread: growth curve of cells HEK in DMEM

  1. #1 growth curve of cells HEK in DMEM 
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    Hi everyone!

    I need that you help me in this matter if is possible ... someone knows some page where I can find this one particular information, on HEK's behavior cells in the growth media???

    please let me know

    THANKS! :roll:


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  3. #2  
    Forum Cosmic Wizard paralith's Avatar
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    do a search on Pubmed, or go to the website of the company or cell bank you got them from.


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  4. #3  
    Forum Masters Degree samcdkey's Avatar
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    Run a growth curve assay.
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  5. #4  
    Forum Cosmic Wizard spuriousmonkey's Avatar
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    how do you do that?
    "Kill them all and let God sort them out."

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  6. #5  
    Forum Masters Degree samcdkey's Avatar
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    The way I do it is to seed the same number cells in growth media (according to manufacturers instructions) in several well plates and count the cells in one well every hour for 24 to 48 hours (depending on confluence). You can make it more accurate by using duplicates, depends on your skill.

    Graph it for the growth curve.
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  7. #6  
    Forum Cosmic Wizard spuriousmonkey's Avatar
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    oh...

    sounds solid.
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  8. #7  
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    THANKS

    :wink:
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  9. #8  
    Forum Masters Degree samcdkey's Avatar
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    Another thing to remember is that if many people share your incubator and open it often, the CO2 level can drop several times and this affects the cells. So if I use an incubator or work in a different lab I repeat the assay as simple things like maintenance of incubator and CO2 levels can have tremendous impact.

    I also note down odd stuff like time of day, CO2 level and temp, if it varies from the norm or I miss a timepoint, in my notebook. Tremendously useful in case you get stuck at some point.
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