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Thread: Chromosomes: simple

  1. #1 Chromosomes: simple 
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    what is the morphology of the chromosome as seen with a light microscope?


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    Universal Mind John Galt's Avatar
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    Small.


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    Forum Cosmic Wizard spuriousmonkey's Avatar
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    You can only see it under special circumstances. And it's morphology depends on the cell cycle.
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    Quote Originally Posted by spuriousmonkey
    You can only see it under special circumstances. And it's morphology depends on the cell cycle.
    Exactly. What if we take the prophase as the default stage? Is the morphology of the chromosomes called karyotype?
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    It probably depends on the preparation?

    My recollection is yes, but I would not put any money on that.

    Here are some images:

    http://www.nature.com/emboj/journal/...7600146f6.html
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    Quote Originally Posted by free radical
    It probably depends on the preparation?

    My recollection is yes, but I would not put any money on that.

    Here are some images:

    http://www.nature.com/emboj/journal/...7600146f6.html
    I need somebody who is a bit sure about karyotype.
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    My post was primarily intended to provide the images. Google is a wonderful tool.

    Nonetheless, my recollection from classes 25 years ago is that karyotypes were based on light microscopy. In fact, karyotypes have been around for a fair while, which might be a point in favour of these being a result of light microscopy.

    Some areas of the chromosome are denser than others (depending on the number of histones present and other factors) thus resulting in the banding pattern you are thinking of. Nevertheless, the type of staining one uses on the preparation will affect how the chromosomes appear.

    Perhaps someone with more recent knowledge will come along to provide a more concrete answer for you.
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  10. #9  
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    Quote Originally Posted by ritwik07
    Quote Originally Posted by spuriousmonkey
    You can only see it under special circumstances. And it's morphology depends on the cell cycle.
    Exactly. What if we take the prophase as the default stage? Is the morphology of the chromosomes called karyotype?
    As far as I know a karyotype is basically a view of the chromosomes, paired, laid out neatly, and labeled. It can be used to detect genetic defects such as trisomy 21 or Turner or Klinefelter's Syndrome.
    "There is a kind of lazy pleasure in useless and out-of-the-way erudition." -Jorge Luis Borges
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