Thread: i have a Question about the DNA Replication

What are the possible attachment sites for a new nucleotide to the ends of an existing polynucleotide? Why doesn’t it matter if the primer for DNA synthesis is either a segment of RNA or DNA?

thanks

What do you mean attachment sites are you talking about WC pairing or 5'-3' phosphodiester bonds?

I think it does matter if the primer is RNA or DNA as a primer which is RNA will have to be replaced by DNA by DNA polymerase III (I think)

i meant by the attachment sites 5'-3' phosphodiester bonds...
thanks for the response.

i have got this Question...If the temperature for melting the DNA is generally above 75 degrees C, what happens to normal DNA polymerase at that temperature?

It's an enzyme, it would have denatured before reaching this point, also there is far more to DNA replication than simply the presence of DNA polymerase (if thats what you're getting at)

I think they are getting more towards PCR .... Taq Polymerase is great!

thanks

On Transcriptional Modification i got this Question:

If cytoplasmic mRNA from a eukaryote were allowed to form a hydrogen-bonded RNA-DNA hybrid structure with the melted chromosome from the same organism, would it be possible to identify the introns? On which strand would you expect to see loops of single stranded nucleotides?

Are these homework questions? I am beginning to get the hint that they are. Sorry to butt in. Just bringing this to light.

That is pretty clearly a homework question....

Just draw it out. This isn't a homework place.

those Questions are not a homework.... im taking online Biology course and these kind of Question are thinking Questions foe each Module...

thanks any way