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Thread: Yeast Bioreactor

  1. #1 Yeast Bioreactor 
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    Hello one and all,
    I am looking for any advice, guidance or pointers surrounding the topic of a DIY bioreactor. The bioreactor does not need to be industrial size or grade and to contextualise, would be used to grow and harvest enough recombinant insulin producing yeast to produce enough insulin for an individual or small community containing individuals with type 1 diabetes. No small contribution or tid-bit of info would go unappreciated as I am beginning here with a lot of knowledge around biology/biotech but little-to-no knowledge of engineering. Materials, pumps, internal/external pressures, filters, nutrient mixtures, filtration processes post cultivation, time-scales, energy requirements. Literally anything provided would be greatly appreciated.
    Sincerely
    OJ


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  3. #2  
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    Quote Originally Posted by hodki087215 View Post
    Hello one and all,
    I am looking for any advice, guidance or pointers surrounding the topic of a DIY bioreactor. produce enough insulin for an individual or small community
    This is going to be way beyond the ability of a novice. If you have access 5 to 10 million dollars (or more) you may be able to cobble something together that won't kill you, maybe. I doubt you could ever get your insulin approved by FDA.


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  4. #3  
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    This isn't for production or profit. It's for a whatif concept. 5 to 10 million dollars or more is a ridiculous amount to talk about all things considered. I've managed to find a source of recombinant yeast to seed an initial culture for US $260. The tank itself and pumps should be fairly cheap when you consider the simplicity of what they're doing. I have no idea about mediums/nutrients as of yet but I would imagine the most expensive part of a small-scale bioreactor not designed for commercial production would be the energy costs.
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  5. #4  
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    Quote Originally Posted by hodki087215 View Post
    This isn't for production or profit. It's for a whatif concept. 5 to 10 million dollars or more is a ridiculous amount to talk about all things considered. I've managed to find a source of recombinant yeast to seed an initial culture for US $260. The tank itself and pumps should be fairly cheap when you consider the simplicity of what they're doing. I have no idea about mediums/nutrients as of yet but I would imagine the most expensive part of a small-scale bioreactor not designed for commercial production would be the energy costs.
    This reply is pretty compelling evidence that you are not aware of the complexity and degree of difficulty of making insulin.
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  6. #5  
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    Your replies to both of my posts in two separate forum areas is "compelling evidence" you have some sort of personal vendetta against sharing knowledge and ideas on the specific topic of insulin production or perhaps simply cultivating yeast. But since you're apparently so much more aware than me of what a complex task harvesting recombinant yeast with the end goal of producing viable short acting insulin is, perhaps share some of your insights? I mean, I figured it would be REALLY simple, I was mostly just making these posts to kill some time before I begin administering a home-grown batch I prepared earlier to the wider community. Please, enlighten me.
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  7. #6  
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    Could it be something to do with PH balances? Relative pressures? Perhaps the effect of light or the metals used and how they interact with the yeast? The composition of the gaseous sections of the system. The potential corrosive effect of constituents and by-products? The re-usability and shelf-life of the components themselves, Temperature? Waste disposal? The purification processes? Nutrient requirements? Storage conditions for the insulin? Additives for long-term storage? Would some sort of automated agitation of the culture be beneficial? Or is a static system preferable? Gosh, the staggering amount of variables and factors involved make me wish I could just enjoy a thought experiment involving play-dough or a an electric toilet-roll dispenser. You're right, better just to quit thinking about it altogether....
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  8. #7  
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    Quote Originally Posted by hodki087215 View Post
    Could it be something to do with PH balances? Relative pressures? Perhaps the effect of light or the metals used and how they interact with the yeast? The composition of the gaseous sections of the system. The potential corrosive effect of constituents and by-products? The re-usability and shelf-life of the components themselves, Temperature? Waste disposal? The purification processes? Nutrient requirements? Storage conditions for the insulin? Additives for long-term storage? Would some sort of automated agitation of the culture be beneficial? Or is a static system preferable? Gosh, the staggering amount of variables and factors involved make me wish I could just enjoy a thought experiment involving play-dough or a an electric toilet-roll dispenser. You're right, better just to quit thinking about it altogether....
    Nobody here is an expert on pharmaceutical manufacturing. From what I gather from a quick web search, you need to brew the yeast, somehow centrifuge it to separate the proteins, and then put them through some kind of chromatographic process to separate out the insulin.

    I suggest you start by talking to these people: https://openinsulin.org. whose mission seems to be to make low-cost insulin available in the USA to people who feel they are being gouged by Big Pharma for what they need.

    As for "vendettas" against sharing information, this is deranged conspiracy talk. Are you a Trump voter?
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  9. #8  
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    Thanks for the pointer exchemist. You'd have to read the back and forth between myself and the gentleman above on the two threads I made to understand my frustration with him. I don't actually believe he has a vendetta lmaooo. Thankyou for at least contributing something, I'm not posting because I feel I'm being "gouged by Big Pharma" although the free health service that provides my insulin probably is. It really is more a thought experiment at this stage than anything else, all I'm actually trying to gain is some knowledge (as much as possible) that I don't have, so I'm researching things but thought it would be worth posting and seeing what an online community of scientists and science enthusiasts might provide. No I'm not a trump voter hahaha, I don't live in America... Makes for some interesting viewing from across the pond though...I'm not jealous of you and your people. I hope things work out in the end
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  10. #9  
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    Quote Originally Posted by hodki087215 View Post
    Thanks for the pointer exchemist. You'd have to read the back and forth between myself and the gentleman above on the two threads I made to understand my frustration with him. I don't actually believe he has a vendetta lmaooo. Thankyou for at least contributing something, I'm not posting because I feel I'm being "gouged by Big Pharma" although the free health service that provides my insulin probably is. It really is more a thought experiment at this stage than anything else, all I'm actually trying to gain is some knowledge (as much as possible) that I don't have, so I'm researching things but thought it would be worth posting and seeing what an online community of scientists and science enthusiasts might provide. No I'm not a trump voter hahaha, I don't live in America... Makes for some interesting viewing from across the pond though...I'm not jealous of you and your people. I hope things work out in the end
    For what it's worth, I'm in London.

    Anyway I hope the Open Insulin people can give you some useful information.
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