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Thread: Prevent growth fungi

  1. #1 Prevent growth fungi 
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    Hello,

    I'm currently working on the isolation and identification of microorganisms from wooden barrels.
    I'm just interested in yeasts and bacteria, but I mostly get a very large growth of fungi (mold).
    Does anyone know what product I can add to my soil (solid and liquid) to prevent the growth of fungi?

    Greetings


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  3. #2  
    Moderator Moderator Cogito Ergo Sum's Avatar
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    Quote Originally Posted by biochemist View Post
    Hello,

    I'm currently working on the isolation and identification of microorganisms from wooden barrels.
    I'm just interested in yeasts and bacteria, but I mostly get a very large growth of fungi (mold).
    Does anyone know what product I can add to my soil (solid and liquid) to prevent the growth of fungi?

    Greetings

    Have you tried adding antifungal substances (e.g. allylamines, azoles, etc.) to your medium?


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  4. #3  
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    Yh, but those antifungal substances also kill the yeasts, because they are very simular.
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  5. #4  
    Forum Cosmic Wizard
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    I am not a microbiologist but I have a few questions.
    Can you separate the cultures manually?
    What about separating the cultures out by differential mediums?
    Maybe the fungi are more sensitive to alkalinity, alcohol concentrations, or CO2 levels than what you are trying to culture.
    Would culturing in a liquid medium let you separate the fungi out easier?
    If I remember correctly from brewing instructions, yeast tends to sink instead of float while most other fungi tend to form mats.
    Can you use different lighting or temperatures to influence what grows?

    Could you grow some culture with just the bacteria and some with just the fungi and then separate the yeasts from the other molds.
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  6. #5  
    Forum Professor Zwolver's Avatar
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    Yeah there are plenty things you could do for this, however most are pretty radical.

    Add SiDNA to prevent growth of mold you don't want to grow. It's extremely specific, and you can make it work on anything.

    You could add proteins that destroys adhesion proteins, so mold won't be able to form constructs. This will slow down growth, but won't kill the mold.

    Add antibodies to mark, and neutralize the mold.

    Otherwise you have to play with salt concentrations, acidity, and oxygen on how to slow down the growth of this mold.

    Hope this was helpfull..
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