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Thread: Mitochondrial Research

  1. #1 Mitochondrial Research 
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    Hello everybody,

    My question is this: Imagine that you guys had a mitochondria(s) and a recently new discovered compound. How would you do to see if this new compound acts within the mitochondria? How you would know if this compound is, for example, inhibiting the electron transport chain? or destroying mitochondrial membranes? How to see if the compound acts or not in the mitochondria.

    Thanks


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  3. #2  
    Forum Professor Zwolver's Avatar
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    Question is to broad.

    It could do anything.


    Growing up, i marveled at star-trek's science, and ignored the perfect society. Now, i try to ignore their science, and marvel at the society.

    Imagine, being able to create matter out of thin air, and not coming up with using drones for boarding hostile ships. Or using drones to defend your own ship. Heck, using drones to block energy attacks, counterattack or for surveillance. Unless, of course, they are nano-machines in your blood, which is a billion times more complex..
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  4. #3  
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    Ok what about the metabolism? How would you study the metabolism factor in mitochondria like Krebs Cycle, Glycolise etc..?
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    Forum Professor Zwolver's Avatar
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    I could think of several ways how you could monitor this. However i have not used any of these techniques.

    Using labelled sugar molecules (using radioactive carbon). Then you could see where it ends up in the cell. However this is not specific for mitochondria.

    Another way is, to suspend the mitochondria in medium, or cell broth. Then see what the products per time unit would be from this broth. You can use several compounds in this broth, but the mitochondria die very quickly outside the cell (+/- 20 hours).

    Last way i could think off, and probably the most obvious one. Simply grow the cells, and test the whole cells on medium. Adding compounds, and monitoring growth and RNA/cDNA levels per protein, used in mitochondria..

    There should be other ways, but far more farfetched then my ideas..
    mikepotter84 likes this.
    Growing up, i marveled at star-trek's science, and ignored the perfect society. Now, i try to ignore their science, and marvel at the society.

    Imagine, being able to create matter out of thin air, and not coming up with using drones for boarding hostile ships. Or using drones to defend your own ship. Heck, using drones to block energy attacks, counterattack or for surveillance. Unless, of course, they are nano-machines in your blood, which is a billion times more complex..
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  6. #5  
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    You can also purify mitochondria and run various tests on them directly. I'm not expert on that, but I know it is done fairly routinely.
    Cogito Ergo Sum likes this.
    Care to read some more of what I have to say? Check out my blog!
    http://wwwscienceisforeveryone.net
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