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Thread: Sufficient stain for an experiment?

  1. #1 Sufficient stain for an experiment? 
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    WARNING: My question probably requires knowledge of a research technician or above, but if you can help..thank you!

    Hi,

    I was wondering if you could help me with a part of my project, Project Design in Physiology. For the purposes of the project I am required to prepare a grant application and as such I need to know the costings of particular materials, such as mice, materials and equipment.
    My hypothesis is about the formation of atherosclerosis in mice who have had a certain gene knockout. Because of this, one of my proposed experimental methods includes staining techniques and I so I was wondering if someone could help me with regards to the quantity of stain required? I'm using the stains haematoxylin, Oil Red O, Sudan IV and fast green. I propose to stain the hearts and aorta of 90 mice but I'm completely lost as to how much stain this means I need. The distributors seem to list the stains in grams of powder, and I can't find out how much liquid stain say 25g of haematoxylin will make up and if it will be sufficient. Would anyone know how much of each stain would be sufficient for the proposed number of mice?
    I'm sorry to take up your time and I know it's a lot to ask, but I would appreciate any help or being pointed in the right direction.

    Thank you and happy new year,
    Ray



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