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Thread: How to predict speed of lysis in a red blood cell solution?

  1. #1 How to predict speed of lysis in a red blood cell solution? 
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    Blood was added to 12 chemicals. I predicted that the chemicals with a higher molecular weight would lyse slower than those of a smaller molecular weight. My results disproved that. The numbers where all over the place. What factor allows you to predict what will lyse quicky and what will lyse slowly. Please explain in detail. Thank you


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  3. #2  
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    homework? If so we don't give away answers here. Show us you did some thinking.


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  4. #3  
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    No not homework, lab analysis. Secondly my original post tells you that I considered molecular weight. I know about hypotonic vs hypertonic, but the question i'm asking is basically: is there a way to know a chemical will create a hypotonic environment for red blood cells before the experiment?
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  5. #4  
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    which chemicals?
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  6. #5  
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    glucose, sucrose, ethanol, methanol, ethylene glycol, glycerol, glycine, sodium chloride, ammonium chloride, potassium chloride, ammonium acetate, sodium acetate these were the chemicals
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  7. #6  
    SHF
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    why did you make your original prediction? based just on weight? There are many other relevant parameters.

    This paper will tell you a bit about lysis with ammonium chloride:
    http://www.science.uva.nl/research/s...yshev2008a.pdf


    This link will give you some ideas about the basic physics of RBC lysis and a couple of examples:
    Lysis of Erythrocytes
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