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Thread: PAGE gel

  1. #1 PAGE gel 
    Forum Freshman
    Join Date
    Jun 2012
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    8
    guys my gel is very weak. When I am going to irradiate it, it breaks into peaces.

    Any ideas to solve this problem?

    thks
    Ana


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  3. #2  
    Forum Professor Zwolver's Avatar
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    Use the correct amounts of bisulfide and polyacrylamide.

    You could use higher concentrations of both and see if it still works right.

    And you could boil the gel longer, or make it cast slower. This will provide a denser structure.

    Also using a tray, and never taking it out of the tray, will make it practically certain it won't break.

    Hope this helps..


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  4. #3  
    Forum Bachelors Degree
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    Jul 2008
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    420
    Increase the % of the gel

    Best wishes,

    Tri
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