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Thread: Lateral Flow Assay: HELP!

  1. #1  
    Comet Dust Collector Moderator
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    It's poor form to post the same thing in two different places. This seems like a chemistry question; you should have just kept it there.


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  3. #2  
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    Sorry, I have no experience in forums and I guess I am learning the etiquette behind it all now.


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  4. #3  
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    It seems well placed to me, given that the topic is pertinent to Biochemistry and Molecular Biology. I look forward to more of your posts, knclayton, you are welcome here. And i will post on your lateral flow assay problem, once i have got my head around it...

    Best wishes,

    Tri
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  5. #4  
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    Hi knclayton,

    The following Nature Protocols paper would probably address some of your questions, unfortunately I am unable to access the full text: http://www.nature.com/nprot/journal/v1/n1/abs/nprot.2006.38.html

    Also, are you using lateral flow test strips? In this case, Millipore provide technical advice here: http://www.millipore.com/publications.nsf/a73664f9f981af8c852569b9005b4eee/348ee7096d93729b85256bf40066a40d/$FILE/tb500en00.pdf (there will probably be similar guides available for other commercial suppliers)

    1. Notably, the Millipore guide suggests that the nitrocellulose membrane can be wet.

    2. I would think that borate refers to Boric acid?

    3.
    I don’t know, sorry

    Are you sure that the concentration of both VEGF sample and anti-VEGF Ab/gold colloid particle are sufficiently high to allow detection?

    Best wishes,

    Tridimity
    Last edited by tridimity; March 9th, 2012 at 12:09 PM. Reason: numbers fudged up
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