Thread: Long-term storage of purified RNA

Greetings Forum Community,

I am searching for information on the best ways to store total RNA isolated from fish tissue for a long time. I have found many recommendations for storage up to one year, but I am hoping to find a method that will work "indefinitely." The best sounding method was to precipitate the RNA in EtOH and store in -80^oC. I have read that this method is good for up to one year. Does anyone have experience with longer-term storage of RNA, or does anyone have knowledge about storing RNA. Any help would be greatly appreciated.

Thanks!

Heya,

My Lab use RNA-Later; RNA samples can be stored at -20*C or below in RNA-Later, indefinitely. Available from Applied Biosystems

BW,

Tri~

Hi Tri,

Thanks for the response. I have another question. Do you store your RNA as a precipitated pellet or in solution?

Thanks.

Hiya,

Normally when I extract RNA from tissue samples I use it immediately in the generation of cDNA for subsequent use in qRT-PCR and use TriReagent for the isolation of RNA, so have never used RNA-Later personally. However, I would imagine that the RNA would be stored in solution.

BW,

Tri~