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Thread: Long-term storage of purified RNA

  1. #1 Long-term storage of purified RNA 
    New Member
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    Sep 2011
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    Greetings Forum Community,

    I am searching for information on the best ways to store total RNA isolated from fish tissue for a long time. I have found many recommendations for storage up to one year, but I am hoping to find a method that will work "indefinitely." The best sounding method was to precipitate the RNA in EtOH and store in -80oC. I have read that this method is good for up to one year. Does anyone have experience with longer-term storage of RNA, or does anyone have knowledge about storing RNA. Any help would be greatly appreciated.

    Thanks!


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  3. #2  
    Forum Bachelors Degree
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    Heya,

    My Lab use RNA-Later; RNA samples can be stored at -20*C or below in RNA-Later, indefinitely. Available from Applied Biosystems

    BW,

    Tri~


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  4. #3  
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    Hi Tri,

    Thanks for the response. I have another question. Do you store your RNA as a precipitated pellet or in solution?

    Thanks.
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  5. #4  
    Forum Bachelors Degree
    Join Date
    Jul 2008
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    Hiya,

    Normally when I extract RNA from tissue samples I use it immediately in the generation of cDNA for subsequent use in qRT-PCR and use TriReagent for the isolation of RNA, so have never used RNA-Later personally. However, I would imagine that the RNA would be stored in solution.

    BW,

    Tri~
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