Thread: Protein assay

Hi everyone

Im trying to do a immunoprecipitation, and I have a doubt the concentration of protein that is obtained after to do the lysate of cells in each well of a plaque of 6 wells. Cause I need to know the amount of buffer lysis that is required

thanks!

What I get protein concentration in each well?

Hi kitty,

You could carry out a BCA assay in order to determine the protein concentrations in each well.

http://en.wikipedia.org/wiki/Bicinchoninic_acid_assay

Best wishes,

Tridimity