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Thread: Cell Cycle Proteins

  1. #1 Cell Cycle Proteins 
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    My first question:

    I have exhausted google and still have not been able to find the answer to this question, so maybe some genius on here can help? Here is a summary of the problem

    A mutation causes a cell to gradually decrease in volume over a series of cell cycles. Analysis of the proteins in G1 and G2 using SDS-page shows an abnormal protein of 90kDa in weight. The wild type is 100kDa.What protein could this be and could you suggest its activity?

    Sorry for asking a uni related question, but as I said, I really have no idea!

    Thanks


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  3. #2  
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    My best guess would be Wee1, although I think this is normally 96kDa in size rather than 100kDa.

    Cheers,

    Tridimity


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  4. #3  
    Forum Cosmic Wizard i_feel_tiredsleepy's Avatar
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    Ya, a non-functional wee1 gene results in wee cells (hence its name). Without it a cell will enter mitosis early. (Also, a 100 kDa band on an SDS-PAGE could correspond to wee1, it is the only likely answer).

    The only other protein that could cause smaller sizes that I know of is cdc25 and it needs to be overexpressed, unlikely to happen from missing a section of it, and is also only 65 kDa.
    Last edited by i_feel_tiredsleepy; August 3rd, 2011 at 01:45 AM.
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  5. #4  
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    Damn, I feel like an idiot now. I go to Edinburgh university. Those sneaky so and so's, Wee1 was discovered here.

    Thanks to both of you!
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