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Thread: flow cytometry

  1. #1 flow cytometry 
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    any one here have done flow cytometry experiments?


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  3. #2  
    Moderator Moderator TheBiologista's Avatar
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    Yes.


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  4. #3  
    Moderator Moderator TheBiologista's Avatar
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    I've used PI but i don't know of the method you mentioned.
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  5. #4 flow cytometry 
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    are you familiar with the protocol of Nicoletti or at least, do you use propidium iodide as your stain?
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  6. #5 flow cytometry 
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    the protocol requires the cells to be harvested, washed twice with cold PBS through centrifugation, and resuspend the cells in a hypotonic fluorochrome solution with PI overnight in the dark at 4oC.
    how long did you incubate your cells with PI? did you use adherent cells
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