Thread: flow cytometry

any one here have done flow cytometry experiments?

Yes.

I've used PI but i don't know of the method you mentioned.

are you familiar with the protocol of Nicoletti or at least, do you use propidium iodide as your stain?

the protocol requires the cells to be harvested, washed twice with cold PBS through centrifugation, and resuspend the cells in a hypotonic fluorochrome solution with PI overnight in the dark at 4oC.
how long did you incubate your cells with PI? did you use adherent cells