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Thread: Laboratory techniques

  1. #1 Laboratory techniques 
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    Mar 2011
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    Hey everyone!,

    I have been trying to figure out what the authors of this paper are doing in terms of their methods- I have to understand the methods so I can produce a condensed transcript of the paper. My tutor has been pretty useless with help, so I though maybe someone on here could help :-D

    Here's a link
    http://onlinelibrary.wiley.com/doi/1...08.07049.x/pdf

    Here's the reference
    Maiso, P. et al. 2007. The insulin-like growth factor-I receptor inhibitor NVP-AEW541 provokes cell cycle arrest and apoptosis in multiple myeloma cells. British Journal of Haematology. 141, 470–482

    My interpretation so far:
    Cell proliferation, cell cycle and apoptosis assays- They are working out whether the drug slows/stops cell growth

    Microarray RNA analyses- To find out when the beginning of apoptosis is, and extract the total RNA at this point-but I don’t know why

    Immunoprecipitation and Western blotting- not sure what they’re trying to extract and analyse here.

    Subcellular fractionation- Again, not too sure here.

    Effect of interleukin-6, IGF-I and BMSC on NVP-AEW541-induced growth inhibition- This is to see what effect these chemicals have on NVP-AEW541

    In vitro kinase assay- an in vitro test on the effects of NVP-AEW541 on the MM cells

    MM xenografts- An in vivo test on a mouse

    Any help on why the authors are doing what they are doing would be greatly appreciated!

    Thanks!


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