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Thread: Wonder if anyone can help me? (Immunological- NK Cells)

  1. #1 Wonder if anyone can help me? (Immunological- NK Cells) 
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    Hello there,

    I am currently undertaking an experiment into the effects of a certain chemokine on NK Cells. I have undertaken a transmigration assay a number of times and have concluded that the NK Cells will migrate in response to this chemokine however i am debating where i should go next as i am not sure on a follow up experimental process that would allow me to look at the effect of the chemokine in another light...
    My colleges suggested using some form of flow cytometry but i feel i wouldn’t be achieving anything if i were to do this.
    I am just wondering if anyone would have any suggestions for me?
    Thank you :-D


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  3. #2 Re: Wonder if anyone can help me? (Immunological- NK Cells) 
    Moderator Moderator TheBiologista's Avatar
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    Quote Originally Posted by kaylz-hughes
    Hello there,

    I am currently undertaking an experiment into the effects of a certain chemokine on NK Cells. I have undertaken a transmigration assay a number of times and have concluded that the NK Cells will migrate in response to this chemokine however i am debating where i should go next as i am not sure on a follow up experimental process that would allow me to look at the effect of the chemokine in another light...
    My colleges suggested using some form of flow cytometry but i feel i wouldn’t be achieving anything if i were to do this.
    I am just wondering if anyone would have any suggestions for me?
    Thank you :-D
    Depends entirely on what sort of "effects" your looking for. What is your hypothesis?


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  4. #3  
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    Sounds exciting! As to what you next investigate, I guess it depends on the degree of freedom that you are allowed in the Project - are you allowed to pursue your own lines of interest, or are you expected to simply follow your superiors' experimental plans? Flow cytometry has been suggested as the next experimental technique, what question could be addressed using this approach?

    Otherwise, it's really exciting that you see this reproducible migration. How much is currently known as to the mechanism by which the chemokine has this effect on NK cell migration? That is arguably the crux of the matter. Once the mechanism has been elucidated, it may be possible to modulate the activity of components of the system, such that you can manipulate NK cell migration. This, in turn, could potentially have significance in the understanding and treatment of immunological disorders and/or cancer etc. Also, would it be possible to use in vivo models to investigate the significance of the transmigration further?

    God I wish I could work on something this interesting.
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  5. #4  
    Moderator Moderator TheBiologista's Avatar
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    Might also make sense to further confirm the specificity of the chemotaxis before proceeding further. What happens if you block the assumed receptor(s) for chemokine X?
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