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Thread: standard curve for qPCR

  1. #1 standard curve for qPCR 
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    i'm planning to do a relative quantification and from what i know, i have to perform standard curve for each genes that i used in qPCR. actually, i have about 25 genes of interest. so, do i really need to perform the standard curve for 25 genes??


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  3. #2  
    Forum Freshman LordKelvin's Avatar
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    Yes, since your Ct's will depend not only on the relative amount of gene copies in your sample but also on other factors such as Tm of primers, product length etc, which are unique to each primers-target combination.

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    Just Because Something's Unexplained Doesn't Mean It's Supernatural - Houdini
    Quantitative PCR
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  4. #3  
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    Definitely: plus sequence and structure-related issues for each amplified region, I would bet.
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