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Thread: How to DIY Agar to grow bacteria and fungus

  1. #1 How to DIY Agar to grow bacteria and fungus 
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    what should i add into agar mix for me to for bacteria and fungus?


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    Forum Freshman Trillian's Avatar
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    Hello, I am new to this forum and just saw your question. Are you utilizing an agar lined plate? If so, then you do not need to add anything; just streak it with your specimen. If you're mixing your own agar nutrient, then give me a list of what you already have and I'll let you know what else you may need. Is this for a science fair or a school project?


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    Forum Cosmic Wizard i_feel_tiredsleepy's Avatar
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    You can find the standard Agar mixes all over google, different additives and % of Agar are more appropriate for different kinds of micro-organisms, but all that information would be available off google.

    The standard generic additives are tryptone, glucose or dextrose, and yeast extract though. Digested soy protein is used a lot too. Some electrolytes are a common additive as well.

    Basically you need to provide protein, energy source, and minerals, which ones are appropriate depends on what you want to grow.

    Glucose and yeast extract will be easier to get for a home project, since you can buy both at a grocery store.
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    Forum Freshman Trillian's Avatar
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    i_feel_tiredsleepy is correct and Amazon.com has some great deals on agar mixes, but it depends on the type of bacteria that you are looking to culture. In lab, we use blood agar plates...

    In a pinch and out of boredom, I successfully cultured some unidentified colonies of bacteria from a fingerprint utilizing nothing but a petri dish and a mix of gelatin and sugar, lol. I incubated the dishes in a completely dark environment at around 27C. I have pictures and video of the specimen under 100x and 400x magnification as well as a cool "swarm" video under 40x magnification if anyone is interested in identifying them. This is what I do over winter break!!
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