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Thread: Identifying proteins using proteomics

  1. #1 Identifying proteins using proteomics 
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    Does anyone have any suggestions for how i can identify proteins for a species if they lack a DNA sequence database?

    Also does anyone know of any scientific literature or links for information about a Maldi Tof-Tof machine? :-D


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  3. #2  
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    Good question. I will come back to it, after some reading. :-D


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  4. #3 Re: Identifying proteins using proteomics 
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    Quote Originally Posted by moekecass
    Does anyone have any suggestions for how i can identify proteins for a species if they lack a DNA sequence database?
    Do you have protein sequence? Try BLAST-p.

    http://blast.ncbi.nlm.nih.gov/Blast...._LOC=blasthome
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  5. #4  
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    Dear moekecass,

    There's some general information on mass spectrometry in 'Principles and techniques of practical biochemistry' - 4th Ed. (1994) by Keith Wilson & John M. Walker. Cambridge University Press

    Presumably, there will also be information in any self-respecting molecular biology or biochemistry text.

    Regards,

    Tridimity.
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  6. #5  
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    ...Also, you can use PeptIdent http://www.expasy.org/tools/peptident.html to identify proteins from peptide mass fingerprinting data

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  7. #6 Re: Identifying proteins using proteomics 
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    Proteinguru[dot]com allows to subtract and intersect Mass FingerPrinting data from different experimental groups.
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