Thread: Identifying proteins using proteomics

Does anyone have any suggestions for how i can identify proteins for a species if they lack a DNA sequence database?

Also does anyone know of any scientific literature or links for information about a Maldi Tof-Tof machine? :-D

Good question. I will come back to it, after some reading. :-D

Originally Posted by moekecass

Does anyone have any suggestions for how i can identify proteins for a species if they lack a DNA sequence database?

Do you have protein sequence? Try BLAST-p.

http://blast.ncbi.nlm.nih.gov/Blast...._LOC=blasthome

Dear moekecass,

There's some general information on mass spectrometry in 'Principles and techniques of practical biochemistry' - 4th Ed. (1994) by Keith Wilson & John M. Walker. Cambridge University Press

Presumably, there will also be information in any self-respecting molecular biology or biochemistry text.

Regards,

Tridimity.

...Also, you can use PeptIdent http://www.expasy.org/tools/peptident.html to identify proteins from peptide mass fingerprinting data

Proteinguru[dot]com allows to subtract and intersect Mass FingerPrinting data from different experimental groups.