Notices
Results 1 to 9 of 9

Thread: Agarose gels

  1. #1 Agarose gels 
    Forum Bachelors Degree
    Join Date
    Jul 2008
    Posts
    420
    What's the lowest percent agarose gel that can feasibly be run?

    Tridimity


    Reply With Quote  
     

  2.  
     

  3. #2  
    Moderator Moderator TheBiologista's Avatar
    Join Date
    Aug 2008
    Posts
    2,564
    1-3% weight per volume is about the normal. Not sure how low you can go without the gel becoming watery, though I've heard of 0.7% being used for some applications.


    Reply With Quote  
     

  4. #3  
    Forum Bachelors Degree
    Join Date
    Jul 2008
    Posts
    420
    I ran a 0.6% gel recently, I was just wondering because I would like to be able to distinguish more easily between bands in the 6/7 kb range which have only ~600bp difference...

    Reply With Quote  
     

  5. #4  
    Forum Ph.D. Heinsbergrelatz's Avatar
    Join Date
    Aug 2009
    Location
    Singapore
    Posts
    994
    wait are agarose gels same things as agar jelly the one where you grow cultures?
    Reply With Quote  
     

  6. #5  
    Moderator Moderator TheBiologista's Avatar
    Join Date
    Aug 2008
    Posts
    2,564
    Quote Originally Posted by Heinsbergrelatz
    wait are agarose gels same things as agar jelly the one where you grow cultures?
    Agarose is a purified version of agar, but we're using it in this case for electrophoresis.
    Reply With Quote  
     

  7. #6  
    Forum Professor
    Join Date
    Sep 2007
    Posts
    1,079
    Quote Originally Posted by tridimity
    I ran a 0.6% gel recently, I was just wondering because I would like to be able to distinguish more easily between bands in the 6/7 kb range which have only ~600bp difference...

    Could pour a longer gel than typical, and run very slowly and cold. Some grades of agarose are better at resolution than others.
    Reply With Quote  
     

  8. #7  
    Forum Bachelors Degree
    Join Date
    Jul 2008
    Posts
    420
    Oh, thanks!

    Tridimity
    Reply With Quote  
     

  9. #8  
    New Member
    Join Date
    Feb 2010
    Posts
    2
    Have you tried to run a 0.7% gel with no success?

    I'd probably follow free radical's recommendation - a 0.7% 25cm gel should cut it. If not, maybe go 1%? The fragments aren't that big.
    Reply With Quote  
     

  10. #9  
    Forum Freshman
    Join Date
    Mar 2010
    Posts
    6
    I find 0.8% works very well for this.
    Reply With Quote  
     

Bookmarks
Bookmarks
Posting Permissions
  • You may not post new threads
  • You may not post replies
  • You may not post attachments
  • You may not edit your posts
  •