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Thread: If you were a molecular biologist...?

  1. #1 If you were a molecular biologist...? 
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    If you were a molecular biologist who wanted to know the function of a gene called afs, which way you suppose to study this gene ? Can you explain in few words your plan ?


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    gc
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    For starters I might try knocking out the gene (for example by site-directed mutagenesis or siRNA) and see what happens.


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  4. #3 Re: If you were a molecular biologist...? 
    Universal Mind John Galt's Avatar
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    Quote Originally Posted by vananh2481990
    If you were a molecular biologist who wanted to know the function of a gene called afs, which way you suppose to study this gene ? Can you explain in few words your plan ?
    On the other hand if I wasn't a molecular biologist, but simply wanted help with my homework I would read the forum sticky on homework help and follow its guidelines. It would doubtless work more effectively than the dishonest approach you have used here.

    Just a well intentioned thought.
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    that is a question in my midterm test !
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  6. #5 Re: If you were a molecular biologist...? 
    Forum Cosmic Wizard spuriousmonkey's Avatar
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    Quote Originally Posted by vananh2481990
    If you were a molecular biologist who wanted to know the function of a gene called afs, which way you suppose to study this gene ? Can you explain in few words your plan ?
    For the mouse:

    1. I would study the existing literature.

    2. I would do an expression study on the tissues of interest or the time points of interest. If no specific interest exists, do expression studies on various time points. This can be done in various ways.
    Rough picture: Northern blot of RNA samples of different stages/tissue with Afs probe.
    More specific: in situ hybridization with Afs probe.

    3. generate a construct that would be informative with the expression pattern in mind: conditional, knockout, hypomorph or hypermorph, with specific promotor or not, or in combination with various cre driven promoters.

    4. analyze phenotype.

    5. Microarray between transgenic and wildtype tissue to find targets, or proteomics study.

    6. lots of molecular shit which all depends on previous results.
    "Kill them all and let God sort them out."

    - Arnaud Amalric

    http://spuriousforums.com/index.php
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  7. #6 Re: If you were a molecular biologist...? 
    Forum Sophomore CShark's Avatar
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    Quote Originally Posted by vananh2481990
    If you were a molecular biologist who wanted to know the function of a gene called afs, which way you suppose to study this gene ? Can you explain in few words your plan ?
    What about sequencing the little bugger, then comparing that to know sequences ?
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