Uh, ive isolated this yeast culture from soil and the broth i used to cultivate it in is a glucose yeast peptone broth in a 1 Litre EFlask- i was wondering if anyone could tell me how long i should keep it in the broth, or how to cultivate it using a broth medium. i was planning on just adding a certain amount of broth each day; someone help please :P, i dont know when to tell if the yeast cells are dying out. they formed a sort of biofilm on the sides of the flask after three days...how do i know if the yeasts are dead?