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Thread: Radioactively Labeled ATP

  1. #1 Radioactively Labeled ATP 
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    Hi Everyone,

    Why would it be wrong to use Radioactively labeled ATP to test for the concentration of ATP within the cytoplasm?

    I'm thinking it's because ATP gets broken down and used very quickly.

    Thanks
    Tom


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  3. #2  
    Reptile Dysfunction drowsy turtle's Avatar
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    Thing is, you can't guarrantee it is ATP you're seeing, and not ADP or some other organic molecule containing adenosine/phosphate (depending which part has the radioactive marker). I also don't think it's possible to make ATP using radioisotopes with a suitable half-life to be able to measure it accurately.


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