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Thread: identify the gene that controls the desired characteristic

  1. #1 identify the gene that controls the desired characteristic 
    Forum Freshman Mark Ian's Avatar
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    What kind of tools and methods are used to identify the gene that controls the desired characteristic in plants. I hadn't any luck searching the internet.^^


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  3. #2  
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    Historically, mutagenesis was the tool of choice to identify genes that control a desired trait.


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  4. #3  
    Forum Freshman Mark Ian's Avatar
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    from what I get from wiki and the German wiki, mutagenesis changes the DNA. I need to locate a certain DNA-sequence, then isolate it (not randomly change the DNA).
    thanks for your post

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  5. #4  
    Forum Cosmic Wizard paralith's Avatar
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    What free radical is talking about is targeted knock out of genes. You find some candidate genes, then find ways to effectively turn them off and see what happens.

    Check out this article on identifying genetic determinants. This article focuses on humans and disease genes, but a lot of the theory and methods aren't very different from what you would do in other organisms.
    Man can will nothing unless he has first understood that he must count on no one but himself; that he is alone, abandoned on earth in the midst of his infinite responsibilities, without help, with no other aim than the one he sets himself, with no other destiny than the one he forges for himself on this earth.
    ~Jean-Paul Sartre
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  6. #5  
    Forum Cosmic Wizard i_feel_tiredsleepy's Avatar
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    He did answer your question.

    How it works is you take a plant that reproduces quickly and in large numbers, like arabidopsis, then you generate mutants and see which plants lost the trait. The ones who lost the trait must have had the gene changed, so you can check which genes changed.

    edit: para beat me to it T_T
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  7. #6  
    Forum Freshman Mark Ian's Avatar
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    thanks, that answers my question. (or rather clarifies things)

    paralith, I am unable to open the pdf, would you mind uploading it somewhere?
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